CasOT: a genome-wide Cas9/gRNA off-target searching tool
نویسندگان
چکیده
The CRISPR/Cas or Cas9/guide RNA system is a newly developed, easily engineered and highly effective tool for gene targeting; it has considerable off-target effects in cultured human cells and in several organisms. However, the Cas9/guide RNA target site is too short for existing alignment tools to exhaustively and effectively identify potential off-target sites. CasOT is a local tool designed to find potential off-target sites in any given genome or user-provided sequence, with user-specified types of protospacer adjacent motif, and number of mismatches allowed in the seed and non-seed regions. AVAILABILITY http://eendb.zfgenetics.org/casot/ CONTACT: [email protected] or [email protected] Supplementary Information: Supplementary data are available at Bioinformatics online.
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عنوان ژورنال:
- Bioinformatics
دوره 30 8 شماره
صفحات -
تاریخ انتشار 2014